Science (non) Fiction

Life, science and faith – not necessarily in that order

I gotta have your stamp of approval on my life

OK, so a lot has been going on lately, which is why this has been so woefully un-updated in recent weeks. After my crazy whirlwind summer, including all health crises that have been mostly resolved, I though moving into the fall would slow things down a bit – ha! (though, in other news, did I mention that I splashed a half-liter of boiling water all down my front-side a couple weeks ago in lab? cause I did – apparently I’m becoming accident-prone in my old age – so maybe I can’t blame my knee injury on Peter and the dancing completely :lol: )

However, things are moving forward finally – and, in the biggest news of the past few months, my first first-author paper was accepted last week into a very well-respected peer-reviewed science journal, and it will be published hopefully around the end of the year!!!! The feeling of relief was almost like a physical sensation of something being lifted off my shoulders, especially given how long this thing has felt to be dragging on and on. I’ve already started working on what will hopefully be formed into the basis for my next paper, which needs to be a lot more self-directed, so you could pray about that if you’d like – that I will take the initiative to try things, to suggest things before asking for advice, to brainstorm hypotheses before talking with my boss. That’s kinda the main thrust of a PhD program (becoming an independent researcher), but it’s a kinda slow lesson for me to be learning apparently.

And I’m having to start thinking about what’s really going to happen when I finish my thesis, cause when people ask when I’ll finish, and I say ‘hopefully sometime next year’, well, that’s coming up really fast! And while I feel like I’m ready to be finished with school, I also don’t know that I really feel ready to face the ‘real world’ (I know, I know, suck it up). I’m trying to figure out what my options are for getting some teaching experience while still in school (prayer for that would also be appreciated), or if I need to just wait til I graduate and then stick around and teach for a semester or two before I apply for a full-time teaching position somewhere. And the somewhere is kind of a sticking point as well – I’ve thought about going back to the mountains, or staying around the triad, or maybe heading over towards the triangle (I just spent several days there over the past few weeks, and other than the traffic, it’s really quite nice over there). I don’t know… but I’m going to have to be making some of those decisions soon, and it’s a bit overwhelming…

So there – that’s what’s swimming around in my head right now. We’re working through some really good stuff on prayer in Bible study, maybe I’ll sort through some of that here next time.

I’m a big kid now!

So the trip to Florida for my first big national meeting was a success!
The poster session went really well – everyone was really nice and receptive to the ideas, and most everyone said something like ‘well done’ or ‘good job’ after I’d walked them through the data – it felt really comfortable, which is a first for me, I’m usually really awkward talking about my work, esp with people who know so much more than I do about the field… but it was good, and generated some good discussion/ideas to think about.

And I got to meet some great people – my boss’s old boss (like my ‘grandfather’ in the science world :lol: ), several of his old students (which was great, cause we talk about them all the time and I’d never met them – and they were awesome!), other famous people in the world of my research… so a good trip altogether… I’m still glad it’s over, as it was kinda stressful and exhausting (that’s a lot of lectures to attend all day starting at 8am) – but it was definitely worth it, and I’m kinda excited about spending some time this summer looking at post-docs and other opportunities for post-PhD work!

Under pressure

So in a span of about a week, we’ll be submitting my first ‘first-author’ paper and I’ll be presenting a poster at my first national conference in Orlando :shock: I’m a little overwhelmed by how fast things are moving, after so much slow-moving progress for the last couple weeks/months – I’ve bee perusing the program of posters/presentations for the conference and it’s quite mind-blowing the diversity of research interests represented by the hundreds of participants who will be there. These meetings are generally considered to be a great place to make contacts for future employment, which is also a bit daunting – I’m not exactly sure what the next couple years hold for me career-wise, whether I’m going to persue a traditional post-doctoral fellowship or consider a more ‘alternative’ teaching post-doc, which would allow me to gain more teaching experience while completing a couple years of research as well. Anyway, that just complicates things as far as what I’m looking for in a ‘next step’ – but this conference will hopefully be a great way to see what kind of exciting research is going on elsewhere in the country, at other great schools where I could potentially end up. Plus I just found out that the wonderful lady who let us use her beach house (who is working on her masters in biology at UNCC) will be there presenting a poster as well, so hopefully we’ll be able to get together and compare notes while we’re there :)

Once the paper is officially submitted and then finally accepted, I’ll let you know – in fact, there will be much joyful celebration and maybe even a couple foofy girly drinks to be had! :lol: In the meantime, I’m going to get back to packing for the conference (hard to decide what to wear to meet potential future employers/colleagues)…

Martha Stewart, look out!

I’ve been feeling very domestic lately – who knows why… I was reading Martha Stewart Living while working out in the fitness center this week (yes, I know, but they didn’t have anything else that I hadn’t already read earlier), and they had ‘ligher’ version of comfort foods as a special section, and I got a major hankering for macaroni and cheese – so I made the version from the magazine this night, and it was awesome, if I do say so myself! :) I can’t recall ever having made macaroni and cheese before – and it made tons, so I’ve had homemade lunch and some dinners for the whole week! And now tonight, I made a chocolate cake from scratch, with homemade chocolate frosting, for a housewarming party tomorrow – and though it’s a bit overcooked (hate my oven :( ), I think it’ll still be tasty enough – I also can’t remember the last time I made a cake from scratch – that’s kinda sad from someone who used to love to cook and bake… good news is, it wasn’t really all that much harder to make this one than it was to make the last cake I made from a mix, so maybe it’ll happen more often

In other news, the paper is almost ready – we finally got the updated version from our collaborators, and have made all the changes/additions we need to (I think) – it was a long day Friday with Dan coming to me every few minutes with something else that needed to be added, or that I’d forgotten about – and I’m soooooooo glad that this headaches is almost over! And, when I talked to Dan earlier, he asked about how I felt about authorship of the paper, which had been something I’ve been worrying about – and when I said that I felt like, given the representation of the data in the paper, that even though it pains me, Laurie (grad student up north) should be first author – but then he said, given how much of our work was necessary to get the rest of the figures (ie, we made the mutants and strains used throughout the paper), that we had more work, that the figures weren’t really representative of the amount of work – so I sai, well, ideally, if we could both be listed as co-authors (noted as ‘these authors contributed equally to this work’), that would be best – and he agreed (and said it’s what he wanted to suggest)! Yay! So I feel a lot better about it now, since most of my usable data is going into this paper – and I was worried I wouldn’t get enough credit out of it… so yay! :)

In this week’s episode of ‘Stupid things I Do in the Lab’

I’ve done plenty of stupid things in the lab during my process of learning how to run things, where things are, etc… but it’s usually because I’ve never done whatever experiment before, or I’m trying to change things around to work something new in. This week, however, my big mistake had to do with simple math and remembering things I should be able to easily remember (which is a more embarassing kind of mistake to make, let’s face it). I was doing a Western blot, which is a process of separating proteins on a gel and then applying antibodies that bind to the one protein I want to find. Anyway, when I was applying the primary antibody (there’s a primary antibody that recognizes the protein, and then a secondary antibody that recognizes the primary antibody), I didn’t really think (apparently) and did a 1:10,000 dilution, which is a fairly typical way of doing them. However, what I failed to add into my calculations was that my primary antibody was already diluted 1:100, so I essentially did a 1:1,000,000 dilution. Which resulted in an essentially empty blot at the end of the day, and a very pissed-off me, not understanding what I’d done wrong yet. Fast forward to this morning, in the shower (which is where I have all my important revelations :lol: ), I was thinking what I had to do when I got into work, and I all of a sudden realized what had happened and had a huge ‘d’uh’ moment. So I rectified the situation for today’s blot and, tada, it worked!!! Maybe not giving me the exact answer I was really looking for, but enough to know it worked and I can adjust from here :)

Also, in other work-related news, I got my semester-review from my boss, and while he’s still wanting me to take more ownership of my project (which is something I struggle with, and he’s aware of that), he’s been very pleased with my progress over the past few months, and says he’s seen an improvement in the things he mentioned to me last time – so yay! So I had a good review, and good results – great way to end the week and a good kick-off to a relaxing weekend! :)

The moment that you stepped into the room you took my breath away

So as admitted in a previous post, I am a big fan of Queen’s music – this addiction is enabled by my brother (who, when asked what category Queen belongs in, says, ‘the best band EVER’ :lol: ). So I was listening to my Queen playlist on the way home to Charlotte today, and I was thinking about why I like their music so much. It’s not cause it’s especially profound (any more than any other rock/pop music of the 70s/80s), or that it’s especially good vocal performance (though I do like Freddy Mercury’s voice). I think I like it for the same reason that I like Meatloaf’s music: each song is like a mini-musical, oftentimes encompassing a huge span of emotions or time or life events. Meatload’s ‘Paradise by the dashboard light’ covers the dissolution of a very dysfunctional relationship, and Queen’s ‘Barcelona’ is almost operatic (helped by the fact that Freddy sings with an opera singer :) ) in its soaring phrases and intense emotions. And the brutal honesty of the songs just draws me in and takes me along for the ride – and even if I don’t completely agree with the idea behind some of the songs (I’m thinking of ‘I was born to love you’, which I love, but I somewhat disagree with the whole idea of there being ‘only one person in the whole world for each person’…), I get totally sucked into the emotions of the songs.

I don’t know what this says about my musical tastes, that I can listen to Queen and Meatload and then a minute later listen to Derek Webb or Patty Griffen or Over the Rhine – I’m either well-balanced or schizophrenic – let’s go with the former, ok? :)

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And, finally, I can say that my paper has been submitted for publication in a leading scientific journal!!!!!!!!!! I got the email today from our collaborators and almost fell out of the chair I was so happy – now comes the mind-numbing waiting process, and then the heart-breaking critique from the editors saying what we need to fix/add/remove/completely change… at least it’s off my desk and onto someone else’s! I’m now one step closer to having that elusive DR. in front of my name! :)

I was born to love you…with every single beat of my heart

(I’ve been listening to Queen obsessively lately, hence the post title :) – it really has nothing to do with anything)

Every once in a while, I get a glimpse of the end of the line, the big finish, the grand finale of grad school known as graduation or hooding or thesis defense or getting the heck out of here… today I was talking with my boss at our regularly scheduled weekly meeting and as we were wrapping things up, he casually mentioned that I should, when I have a moment (:lol:), outline my second paper (now that the first one is solidly in the works), what I want to include, what experiments I need to do to show what I want to show, etc… oy! While you’re only really required to have one paper to graduate (a first authorship that is), its much more acceptable to the department (and your boss!) if you have several, or at least some in the works by the time you graduate. Now this brings me no closer to graduating in reality (as its just outlining what I NEED to show for a paper), but its really the first time Dan and I have ever broached the subject (even broadly) of my finishing my PhD work. And this on the day that the first year student who I mentored while she rotated through our lab came and talked to Dan today about her potential projects now that she’s officially chosen our lab for her thesis work! How crazy that I was in that exact same position, scared out of my mind about talking to Dan, just 3 years ago! Some days I feel like I’m right back there at the beginning, that I’ve barely progressed at all, that I’m still making messes everywhere and not understanding anything… and then someone will ask me something, and I’ll think, ‘I actually know the answer to that!’… and then I know that I’m getting closer, that I’m certainly not there yet, but I’m definitely getting closer…

Whew!

Well, I survived my lecture this morning for the first year grad students – they were attentive and asked questions, and my boss told me I did a good job, so I’m pretty happy with the results – especially given that I’d like to teach as a career, it was nice to at least not get a resounding NO as an answer after my first official teaching gig! :) And I’ve got a new haircut, pink roses on my benchtop, Frank Sinatra playing on my computer, and this weekend is going to be great, so I’m a happy, happy girl!
Concert this Friday night in Greensboro at Christ Community Church (http://www.cccarp.org) with Derek Webb, Sandra McCracken (!) and Judd and Maggie (who I don’t know, but are apparently opening for DW and SM) – Matthew will be coming up to spend the weekend with me, so he’s going to try to make the concert too – then we’ll have a fun weekend of movies and eating and maybe we’ll spend some time outside at some point :) too bad we missed the film festival that was running last weekend – we’ll have to make our own I guess…
My dad comes back from WI tomorrow (I think), so it will be nice to have him back – its wierd to call home and not be able to talk to him :( But I’d better get used to it, since he’s recently decided to go back to Kenya again this summer for 3 weeks – which is very exciting! He’s already got all the shots, and the guy who runs the orphanage over there will be going with him, so it will be good for him to see people over there again and see how things have changed – I’d love to go with him, but this summer is going to have to be dedicated to getting some good data under way or my committee is not going to be pleased come fall…
Off to finish work so I can make it to Bible study on time!